mtbc 16s rrna reference sequences  (ATCC)

Journal: Scientific Reports

Article Title: Pulmonary non-tuberculous mycobacteria in colonisation and disease in The Gambia

doi: 10.1038/s41598-022-22777-x

Figure Lengend Snippet: Phylogenetic tree of NTM in The Gambia based on 16S rRNA gene sequence analysis of regions A and B of the mycobacteria gene. Red coloured fonts represent unknown sequences from the present study while fonts in black colour represent NTM and MTBC reference sequences of ATCC strains downloaded from GenBank.

Article Snippet: NTM and MTBC 16S rRNA reference sequences derived from American Type Culture Collection (ATCC) isolates were downloaded from Gen Bank to illustrate the phylogenetic relationship of unknown sequences and identify them.

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Dual color fluorescence in situ hybridization (FISH) assays for detecting Mycobacterium tuberculosis and Mycobacterium avium complexes and related pathogens in cultures

doi: 10.1371/journal.pone.0174989

Figure Lengend Snippet: Results of the MN Genus-MTBC and MTBC-MAC FISH assays with reference Mycobacterium and Nocardia cultures.

Article Snippet: The reference MTBC, two Bacillus subtilis (ATCC 23059 and 6633) and the five Corynebacterium clinical isolate cultures were grown in the Microbiology Laboratory, University of California San Francisco, San Francisco, CA.

Techniques:

Journal: PLoS ONE

Article Title: Dual color fluorescence in situ hybridization (FISH) assays for detecting Mycobacterium tuberculosis and Mycobacterium avium complexes and related pathogens in cultures

doi: 10.1371/journal.pone.0174989

Figure Lengend Snippet: Results of the MN Genus-MTBC and MTBC-MAC FISH assays on reference cultures of pathogens not belonging to the Mycobacterium or Nocardia genera.

Article Snippet: The reference MTBC, two Bacillus subtilis (ATCC 23059 and 6633) and the five Corynebacterium clinical isolate cultures were grown in the Microbiology Laboratory, University of California San Francisco, San Francisco, CA.

Techniques:

Journal: PLoS ONE

Article Title: Dual color fluorescence in situ hybridization (FISH) assays for detecting Mycobacterium tuberculosis and Mycobacterium avium complexes and related pathogens in cultures

doi: 10.1371/journal.pone.0174989

Figure Lengend Snippet: A, B–Dual fluorescence reactivity in the same microscopic field with the MN Genus- and MTBC-specific probes respectively in the MN Genus-MTBC FISH assay; C, D—Dual fluorescence reactivity with the MTBC- and MAC-specific probes respectively in the MTBC-MAC FISH assay. Mycobacteria used in the FISH tests were: LJ culture of M . tuberculosis (ATCC 25177); LJ culture of M . avium (ATCC 25291); MGIT culture of M . kansasii (ATCC 12478); and an artificially mixed LJ culture of M . tuberculosis (ATCC 25177) and M . avium (ATCC 25291). Photographs were taken at x1000 magnification. Scale bars shown in the photographs represent approximately 50 μm .

Article Snippet: The reference MTBC, two Bacillus subtilis (ATCC 23059 and 6633) and the five Corynebacterium clinical isolate cultures were grown in the Microbiology Laboratory, University of California San Francisco, San Francisco, CA.

Techniques: Fluorescence